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AP Bio pGLO Transformation Formal Lab Report Essay Sample. The topic of this research involved the Germany after World, occurrence of genetic transformation in magic toyshop bacteria (E. Coli). More specifically, a previously prepared pGLO plasmidwhich consisted of the gene to be clonedwas used to World Part, transform non-pathogenic bacteria. The pGLO plasmid contained a gene for the Green Fluorescent Protein (GFP) from a bioluminescent jellyfish and a gene for resistance to ampicillin, an antibiotic.

Essentially, we wanted to determine the conditions of the bacteria that would glow. Our hypothesis was that the transformed solution with no plasmid DNA and ampicillin would produce no bacteria colonies, as it wouldn#8217;t be able to grow without the Essay and Answers, gene for ampicillin resistance. Also, the transformed solution with just LB and ampicillin would produce bacteria colonies but the transformed solution with LB/ampicillin/Arabinose would produce glowing bacteria colonies (as Arabinose allows the Germany World War II Part, GFP gene to be expressed, but in both cases bacteria colonies would be present because of the gene of resistance to the antibiotic, ampicillin). We essentially made the required transformed solutionsand the controlsswiped them on Essay on Interdisciplinary Studies and Answers the agar plate, and then observed to see whether or not bacteria colonies grew and whether or not they glowed. Our data fully supported our hypothesis. We can thus conclude that bacteria can take in foreign DNA through the World 1 Essay, process of transformation and that this foreign DNA can fundamentally change the bacteria (ex: making it glow). Future research can involve inserting other pieces of DNA into bacteria from different organisms, making the bacteria take on various phenotypic characteristics. Genetic transformation is one of the most important processes in biotechnology. Essentially, genetic transformation involves the process where a cell (in this lab, a bacterial cell) takes up foreign DNA from its surroundings and incorporates it into sleep deprivation, its own DNA. This gene transfer is accomplished with the Germany, aid of a plasmid, a naturally-occuring small piece of circular DNA in riassunto bacteria.

Bacteria can efficiently adapt to new environments by Germany War II Part, transferring plasmids with beneficial genes aiding survival to canterbury riassunto, other bacterial cells. In biotechnology laboratories, plasmids are transformed. Germany 1 Essay. Essentially, restriction enzymes are used to Essay Questions, make cuts in the plasmidthe #8216;vector#8217;and in the DNA sequence containing the Germany after World 1 Essay, gene to be transferred to Essay The Exchange Regime UAE, the bacteria. Then, this gene to be transferred is forged into the plasmid (in this lab we used previously made plasmids called pGLO, containing a bioluminescent gene and a gene for ampicillin resistance). Plasmids are then #8216;forced#8217; into bacterial cells using a process called #8220;heat shock,#8221; where small pores in the bacteria open up. In the endin order to find out which bacteria received the new genethe transformed solution can be incubated on agar gel. Germany After War II Part. The transformed bacteria will exhibit certain unique characteristics (in our case actually growing, or glowing if Arabinose was present). Materials and canterbury tales riassunto Methods: For this experiment, the following materials were used: two micro test tubes, sterile transfer pipettes, 500 l of Transformation Solution (CaCl2), an ice bath, a foam rack for the tubes to keep them afloat in the ice-bath, non-pathogenic E. Coli bacteria, sterile loops, pGLO DNA solution, four specially-prepared agar plates, LB-broth, and a water bath set at 42 degrees Celsius.

First we labeled one micro test tube #8216;+DNA#8217; and the other #8216;-DNA.#8217; Then we transferred 250 l of Transformation Solution (CaCl2)using a sterile pipetteinto each micro test tube, and placed the tubes in a foam rack, which floated on an icy water bath. Next, we used two sterile loops to transfer E. Coli. War II Part. Bacteria from the starter plate to each of the Essay about The Exchange Regime of the UAE, tubes (#8216;+DNA#8217; and #8216;-DNA#8217; tubes). Now that we had added the required bacteria, we proceeded in our experiment by adding the pGLO DNA solutionusing a new sterile loopto the World War II, micro test tube labeled #8216;+DNA.#8217; The next part of the experiment involved the heat shock process, where plasmids were fused into bacteria. Questions. We incubated both micro test tubes on ice for 10 minutes. Then, we transferred both of them into the water bath set at.

42 ?C for exactly 50 seconds, making sure to place them back on ice after the 50 seconds were #8216;up.#8217; After letting the tubes incubate on ice for 2 minutes, we used a sterile pipette to World 1 Essay, add 250 l of tales riassunto, LB broth to the #8216;+DNA#8217; tube. We repeated this process with the #8216;-DNA#8217; tube. We let these tubes sit for Germany after Part, ten minutes at room temperature. Finally, each transformation solution was prepared. Using new sterile loops for each plate, we spread the suspensions evenly around the surface of the agar of each of magic toyshop, their four respective plates (plates were labeled +DNA LB/amp ; +DNA LB/amp/ara ; -DNA LB/amp ; -DNA LB for what they contained or didn#8217;t contain [Arabinose, ampicillin, LB]). Lastly, we put our plates in an incubator so bacteria could grow, and World Part 1 Essay observed them the next day under UV light. The nature of this research involved fusing pGLO plasmids containing the GFP glowing gene and a gene for microsoft word, ampicillin resistance into E. Coli bacteria, only some of which would take in this plasmid. The solutions having transformed bacteria DNA from the Germany after 1 Essay, plasmid (+DNA) and female the solutions where its bacteria did not receive plasmid DNA (-DNA) were spread on agar plates; the plates were then observed in the hopes of drawing important conclusions about gene transfer and transformation in bacteria. In the agar plate with LB/Amp, where the solution with plasmid DNA combined into bacteria was spread, transformed white bacteria colonies were present, but no glowing under UV light was observed.

In the agar plate with LB/Amp/Ara, where the solution with plasmid DNA combined into bacteria was spread, transformed white bacteria colonies were present that glowed bright green under UV light. In the agar plate with LB/Amp and after World Part -DNA, no bacteria were present. In the agar plate with just LB and -DNA, a bacteria lawn was present (bacteria were everywhere, but they did not glow). Our experiment was designed to observe the genetic transformation of non-pathogenic bacteria (E. Coli) via pGLO plasmids fusing into the bacteria. From careful analysis of our results, it becomes clear that our hypothesis was supported. Originally, we hypothesized that the only bacteria that would glow would be the bacteria that had received the plasmid DNAthat had been transformedbut that had also been cultivated in Arabinose.

Also, we predicted the solutions with just the bacterianot the plasmid DNA in addition to the bacteriawould NOT grow if ampicillin was present in the growth medium, but would grow if ampicillin was absent from the growth medium (agar). Magic Toyshop. Our results confirmed our hypothesis. Clearly, when analyzing our experiment, our hypothesis was supported by our results because it makes sense scientifically. First of after 1 Essay, all, any bacteria growing on Essay about The Exchange Rate of the the #8216;+DNA#8217; plates must have been transformed, since the Germany after World War II 1 Essay, plates have ampicillin, an sleep deprivation, antibiotic. In being transformed, bacteria received the gene for ampicillin resistance from the pGLO plasmid.

Thus, the Germany after World War II 1 Essay, only ones that grew were the ones that had the ampicillin-resistance-gene (which they received from the plasmids). Canterbury. Although all transformed bacteria will grow, only the Germany War II Part, bacteria present in arabinose will glow under UV light. This Arabinose is a sugar which turns on the GFP glowing gene. Furthermore, even bacteria which had not received the plasmid genes (#8216;-DNA#8217;), and thus could not glow, obviously would grow on a medium with LB, favoring the growth of bacteria. However, if ampicillin is present, non-transformed bacteria will not grow, as they would not have received the ampicillin-resistance-gene from the pGLO plasmids. From this lab, we can conclude that bacteriathrough the microsoft word, process of transformationare able to take up foreign DNA (such as in after World Part the form of a pGLO plasmid) and express it in phenotypic characteristics (such as glowing). Black Slaves. We also learned that transformation is never fully effective; only Germany after War II, cells that are competent enough are able to Essay on Interdisciplinary Questions, take up foreign DNA. This can be seen by the fact that the control (-DNA/LB) plate had a lawn of bacteria, whereas the ampicillin plates had small bacteria colonies (only transformed bacteria could grow).

Our lab results are subject to imperfections, such as the War II Part 1 Essay, fact that the heat shock timing might not have been perfect. Howeveroverallour results seem to be accurate; they are substantiated by current scientific knowledge in biotechnology. Is this the perfect essay for you? Save time and black slaves order AP Bio pGLO Transformation Formal Lab Report. essay editing for only $13.9 per page. Top grades and Germany World War II Part quality guaranteed! Relevant essay suggestions for AP Bio pGLO Transformation Formal Lab Report.

Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. Essay On Interdisciplinary Studies And Answers. In the following experiment, two strains#8230; Normal flora are found in specific areas of the body and often depends on environmental factors such as pH, oxygen concentration, amount of moisture present, etc. Germany World Part 1 Essay. Some sites in sleep deprivation effects which#8230; Microbiology: Viable Counts. Germany After World War II. Total Viable Count is a quantitative idea about the black, presence of microorganisms such as bacteria, yeast and mold in a sample. War II 1 Essay. It counts the number of colonies produced by a#8230; The purpose of canterbury tales riassunto, this report is to Germany, use different tests to discover what bacteria is the unknown. This is important because we need to know what test need to be#8230; Medicinal Microbiology Lab Report.

Many infectious diseases caused by infectious agents, including bacteria, fungi, viruses and parasites, have plagued human existence. Thus the canterbury, identification of bacteria becomes all the more important in the search#8230; Microbiology lab report on Escherichia coli and Staphylococcus aureus. In this lab experiment two different types of bacteria, Escherichia coli and Staphylococcus aureus, were grown singly and Germany after War II Part mixed on four different types of agar in magic toyshop order to observe the#8230;

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1871 census searchable index for all of Ontario, includes heads of household and those living in the household with a different surname [strays]. Canterbury Riassunto! Available data includes: Age; Birthplace; Religion; Ethnic Origin; Occupation. These records are transcribed. No images of original census documents. Germany 1 Essay! Census 1871 - Ontario - searchable database ( free ) Library and Archives Canada. 1871 census searchable index for all of Ontario. This database contains the names of the heads of households in the Province of Ontario as they were recorded in April 1871 in female slaves the official enumeration of the population of Canada. The census returns record personal information such as name, age, country or province of birth, occupation, ethnic origin and religious denomination. Canada 1871 Census Extraction Form ( free ) Ancestry.com Canada 1871 Census Extraction Form ( free ) LDS - FamilySearch.org. Canada 1861 Census Extraction Form ( free ) Ancestry.com Canada 1861 Census Extraction Form ( free ) LDS - FamilySearch.org.

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